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Fig. 4 | Cancer Cell International

Fig. 4

From: Short-term starvation synergistically enhances cytotoxicity of Niraparib via Akt/mTOR signaling pathway in ovarian cancer therapy

Fig. 4

SKOV3 and A2780 cells were treated with niraparib and STS alone or in combination. a, b Comet assay in SKOV3 and A2780 cells. We quantified the percentage of DNA in the tails to calculate the DNA damage, and each data point represents 50 cells counted. Scale bar, 20 μM. c, d Immunofluorescence assay in SKOV3 and A2780 cells representing γH2AX and RAD51 foci. Scale bar, 10 μM. e, f Cells were treated with niraparib, STS or their combination, and western blot analysis was used to detect the expression levels of γH2AX and RAD51. GAPDH protein was used as a loading control. The values are presented as the mean ± SD and were analysed using GraphPad Prism (one-way ANOVA). ***P < 0.001, **P < 0.01, *P < 0.05

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