Fig. 6From: Short-term starvation synergistically enhances cytotoxicity of Niraparib via Akt/mTOR signaling pathway in ovarian cancer therapya SKOV3 and A2780 cells were treated with STS, niraparib or in combination, and the ATP level was detected by an ATP assay kit. b A2780 cells were treated with or without niraparib and STS in combination with MK2206 (300 nM) or AZD8055 (80 nM) for 48 h, and cell viability was determined by a CCK-8 assay. c The cells were treated as in Fig. 6b. Phosphorylated Akt (p-Akt), p-mTOR, γH2AX, and RAD51 were detected by western blot. GAPDH protein was used as a loading control. Three independent experiments were performed. ***P < 0.001, **P < 0.01, *P < 0.05Back to article page