Fig. 3

SOX11 is a direct downstream miR-937-3p target in LUAD cells. A Predicating candidate gene targets by intersecting from three different bioinformatics websites (TargetScan, miRDB and miRWalk). B The SOX11 expression level in 60 LUAD tissues and paired adjacent tissues was investigated by qRT-PCR. C The relationship between SOX11 and the survival of LUAD patients (n = 366 in the miR-937-3p-low group and n = 353 in the miR-937-3p-high group). D A negative regulatory effect of miR-937-3p on SOX11 was tested by qRT-PCR. E Predicted miR-937-3p seed region at the 3′- UTR of SOX11. F Luciferase activity was analyzed in LUAD cells co-transfected with miR-937-3p-mimics or negative control with pmirGLO-SOX11-Wt or pmirGLO-SOX11-Mut. G The expression levels of SOX11 protein and AKT pathway related proteins in the transfected A549 and H1395 cells were analyzed by Western blot. 1: NC;2: miR-937-3p;3: inhibitor NC;4: miR-937-3p inhibitor; GAPDH was used as a control. The data expressed as the mean ± SD. (*P < 0.05; **P < 0.01; ***P < 0.001)