Fig. 8

The association between IGF2BP3 and PD-L1. A–C The protein expression levels of IGF2BP3 and PD-L1 in BCa cells were determined by western blotting. T24 (A), 5637 (B) and UMUC3 (C). D–F The mRNA levels of IGF2BP3 and PD-L1 in indicated cells were detected by quantitative polymerase chain reaction (qPCR), Puro was set as 1, Puro vs IGF2BP3, negative control (NC) vs shIGF2BP3. T24 (D), 5637 (E) and UMUC3 (F). G–H Two representative flow cytometry staining of PD-L1 in indicated T24 cells are shown (left), and quantification of PD-L1 fluorescence intensity is shown (right), Puro and NC were set as 1, Puro vs IGF2BP3, NC vs shIGF2BP3. I The mRNA levels of IGF2BP3 and PD-L1 in tumor specimens were detected by qPCR, patient #1 was set as 1. All quantification analyses were based on independent triplicate experiments. Error bars represent the standard deviation (SD). *P < 0.05, **P < 0.01, ***P < 0.001, based on Student’s t test