Fig. 5

The effect of miR-199b-3p on acquired resistance to CTx was mediated by Wnt/β-catenin signaling in CRC. CRC-CTxR cells transfected with miR-199b-3p mimic (miR mimic) or NC mimic; To verify the role of miR-199b-3p in regulating Wnt signaling, miR-199b-3p overexpressing and normal CRC-CTxR cells were exposed to IWR-1-endo (Wnt signaling antagonist). A Western blot detected CRIM1 and the Wnt signaling-related proteins levels in CRC-CTxR cells with different treatments. B CCK-8 assay tested cell viability. C TUNEL assay detected the apoptotic cells. The capacity of cell migration and invasion was evaluated by D wound healing and E transwell assay, respectively. *P < 0.05, **P < 0.01 and ***P < 0.005 compared with control group; ^#P < 0.05, ^##P < 0.01 and ^###P < 0.005 compared with miR mimic group