Fig. 4

Exosomal miR-494-3p is correlated with GOLPH3 expression. Relative fold changes in protein concentrations of exosomal lysates as examined by the BCA assay (a) and NanoSight (b). c Heatmap for differential miRNAs in exosomes between SUN-449 shC and sh1 group. d, e Expressions of differential miRNAs in GOLPH3-downregulated or GOLPH3-overexpressed SNU-449 cells and their corresponding exosomes were validated by qRT-PCR. All experiments were repeated at least 3 times. Error bars represent mean  ±  SD for 3 independent experiments. Data are analyzed by one-way ANOVA with Dunnett’s multiple comparisons test (a, b) or t test (c, d, e). *p  < 0.05; **p  < 0.01; NS not significant