Fig. 7

Exosomal miR-494-3p enhanced angiogenesis and sorafenib-resistance by directly targeting PTEN mRNA. a Target genes for miR-494-3p were predicted by miRDB, miRWalk, miRanda, and TargetScan; intersection gene lists predicted by these algorithms was analyzed using a Venn diagram. b WT and MUT of miR-494-3p binding site on 3′-UTR of PTEN mRNA. c miR-494-3p inhibited the activities of the luciferase reporter that contained the wild-type but not the mutant-type 3′UTR of PTEN. d, f Effects of miR-494-3p overexpression or downregulation on protein levels of PTEN, ρ-AKT and AKT in HUVECs and HCC cells. e, g After miR-494-3p inhibition, effects of exosomes derived from GOLPH3-overexpressed SNU-449 cells on PTEN, ρ-AKT and AKT levels in HUVEC and SNU-449 HCC cells. Data are expressed as mean  ±  SD for 3 independent experiments and analyzed by t test (c). **p  < 0.01