Fig. 3From: DNMT1 facilitates growth of breast cancer by inducing MEG3 hyper-methylationSilencing miR-494-3p restrains progression of breast cancer cells by targeting OTUD4. A DEGs in GSE70905 dataset from GEO database were analyzed; B Venn diagram of DEGs and predicted target genes of miR-494-3p; C differential expression of candidate genes in GSE70905 dataset; D the binding sites of miR-494-3p on OTUD4 3′UTR were analyzed by starBase database; E OTUD4 expression levels in breast cancer cell lines (MDA-MB-231,SUM 149) and normal breast epithelial cell line MCF10A were detected by qRT-PCR; F RIP and G dual luciferase assays were performed to verify targeting relationship between miR-494-3p and OTUD4; H OTUD4 protein expression in NC inhibitor and miR-494-3p inhibitor groups was detected by western blot; I Interference efficiency of sh-OTUD4 was tested by western blot; J miR-494-3p and OTUD4 expression levels, K the cell proliferation, L migration and M invasion in NC inhibitor  +  sh-NC, miR-494-3p inhibitor  +  sh-NC, and miR-494-3p inhibitor +  sh-OTUD4-1, and miR-494-3p inhibitor  +  sh-OTUD4-2 groups were measured. *p  < 0.05Back to article page