Table 1 Primer sequences for qRT-PCR and MSP
From: DNMT1 facilitates growth of breast cancer by inducing MEG3 hyper-methylation
Genes | Primer sequences |
|---|---|
miR-494-3p | F: 5′-GAAACATACACGGGAAAC C-3′ |
R: 5′-GTGCAGGGTCCGAGG T-3′ | |
U6 | F: 5′-CTCGCTTCG GCAGCACA-3′ |
R: 5′-AACGCTTCACGAATTTGC GT-3′ | |
DNMT1 | F: 5′-CGGCTTCAGCACCTCATTTG-3′ |
R: 5′-AGGTCGAGTCGGAATTGCTC-3′ | |
MEG3 | F: 5′-ATCATCCGTCCACCTCCTTGTCTTC-3′ |
R: 5′-GTATGAGCATAGCAAAGGTCAGGGC-3′ | |
MSP-MEG3 (methylation) | F: 5′-TATGAGTTGTAAGCGGTAGAGTTC-3′ |
R: 5′-TACGAACTTAACGAAAAAAAAATCAT-3′ | |
MSP-MEG3 (non-methylation) | F: 5′-GAATATGAGTTGTAAGTGGTAGAGTTT-3′ |
R: 5′-TACAAACTTAACAAAAAAAAATCATACT-3′ | |
OTUD4 | F: 5′-TTCTGATGTGGATTACAGAGGGC-3′ |
R: 5′-ACGCATGTTGTCTTACTCCTGA-3′ | |
GAPDH | F: 5′-GAGTCAACGGATTTGGTCGT-3′ |
R: 5′-TTGATTTTGGAGGGATCTCG-3′ |