Table 1 Antibodies used in the experiment
Primary antibody | Concentration | Company | Second antibody |
|---|---|---|---|
Ebp1 (N-terminus) (WB) | 1:1000 | Millipore | Goat anti-rabbit IgG |
Vimentin (C-20) (WB) | 1:1000 | Santa Cruz | Rabbit anti-goat IgG |
N-cadherin (13A9) (WB) | 1:1000 | Santa Cruz | Goat anti-mouse IgG |
Snail (ab53519) (WB) | 1:1000 | Abcam | Goat anti-rabbit IgG |
E-cadherin (S-17) (WB) | 1:1000 | Santa Cruz | Rabbit anti-goat IgG |
Slug (A-7) (WB) | 1:1000 | Santa Cruz | Goat anti-mouse IgG |
β-catenin (E-5) (WB) | 1:1000 | Santa Cruz | Goat anti-mouse IgG |
p-GSK-3β (Ser 9) (WB) | 1:1000 | Santa Cruz | Rabbit anti-goat IgG |
cyclinD1 (A-12) (WB) | 1:1000 | Santa Cruz | Goat anti-mouse IgG |
β-actin (C4) (WB) | 1:1000 | Santa Cruz | Goat anti-mouse IgG |
Vimentin (C-20) (IF) | 1:200 | Santa Cruz | TRITC-rabbit anti-goat IgG |
E-cadherin (S-17) (IF) | 1:200 | Santa Cruz | TRITC-rabbit anti-goat IgG |