Fig. 2

miR-378c suppresses cell proliferation, invasion and EMT of STAD. qRT-PCR was used to examine the overexpression or down-regulation of miR-378c in MGC-803 or MKN-28 cells (A). STAD cell proliferation was determined by BrdU staining (B, scale bar, 100 μm) and colony formation (C, scale bar, 1 cm) assays. Apoptosis-related proteins were determined by Western blotting (D). Wound healing (E, scale bar, 100 μm) and Transwell (F, scale bar, 100 μm) assays validated the migratory and invasive capacities of STAD cells. The EMT-related proteins were determined by Western blotting (G). *P < 0.05, **P < 0.01, ***P < 0.001. Data represent at least three independent sets of experiment