Fig. 1

SF3A3 is upregulated and correlates with poor survival with bladder cancer patients. A Hematoxylin and eosin staining in tumor and normal bladder tissues. Scale Bar = 25 μm. B Immunofluorescence staining detects the expression of SF3A3 in normal and bladder cancer tissues. Bar = 50 μm. C The SF3A3 protein levels were detected by Western blot in tumor (T) and normal (N) bladder tissues. D Quantitative analysis of protein expression in C. E RT-qPCR was used to detect the mRNA level of SF3A3 in normal (n = 49) and tumor (n = 49) bladder tissues. F The data of SF3A3 expression was analyzed from the TCGA database (http://ualcan.path.uab.edu/analysis.html) in tumor and normal bladder tissues. G The overall survival of BC patients was analyzed from the data of TCGA with low (n = 100) and high (n = 100) SF3A3 levels by Kaplan–Meier analysis (http://www.oncolnc.org/). H SF3A3 protein level was measured by Western blot in BC cell lines (J82, UM-UC-3, 253 J, and T24). I Quantitative analysis of proteins level of H. J RT-qPCR was used to examine the mRNA level of SF3A3 in different cell lines. All data are expressed as mean ± SEM and come from three independent experiments. *P < 0.05, **P < 0.01 vs. the corresponding controls