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Fig. 2 | Cancer Cell International

Fig. 2

From: E2F6/KDM5C promotes SF3A3 expression and bladder cancer progression through a specific hypomethylated DNA promoter

Fig. 2

SF3A3 facilitates the proliferation of bladder cancer cells in vitro. A T24 cells were transfected with pKLO, shSF3A3-1, shSF3A3-2, or J82 with pWPI and oeSF3A3 vectors, and SF3A3 and MYC proteins levels were measured by using Western blot. B Quantitative analysis of protein expression in A. C T24 and J82 cells transfected as A and SF3A3, and MYC mRNA expression was examined by RT-qPCR. D–F T24 and J82 cells were transfected as in A, and then cell viability was explored by MTS (D) and colony formation assays (E, F). All data are expressed as mean ± SEM and come from three independent experiments. *P < 0.05, **P < 0.01 vs. the corresponding controls

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