Fig. 3

Hypomethylation of promoter induces SF3A3 expression by upregulating KDM5C. A The DNA methylation level of SF3A3 promoter was explored from the TCGA database (http://ualcan.path.uab.edu/analysis.html) in tumor and normal bladder tissues. B The GpC island of SF3A3 promoter was analyzed by Methprimer (http://www.urogene.org/cgi-bin/methprimer/methprimer.cgi). C DNA methylation was measured using bisulfite sequencing PCR (BSP) in the GpC island of the SF3A3 promoter. D The expression of candidate DNA demethylases was measured by RT-qPCR in tumor and normal bladder tissues. E T24 cells were transfected with indicated siRNA, and then SF3A3 mRNA was detected by RT-qPCR. F RT-qPCR was used to explore the KDM5C expression in tumor and normal bladder tissues. G Double immunofluorescence staining was used to examine the expression of SF3A3 and KDM5C in tumor and normal bladder tissues. H Quantitative analysis of the fluorescence intensity SF3A3 and KDM5C. I Analysis of the correlation between SF3A3 and KDM5C expression in BC. J The overall survival of BC patients was analyzed with KDM5C levels by Kaplan–Meier analysis from the data of TCGA (http://www.oncolnc.org/). All data are expressed as mean ± SEM and come from three independent experiments. *P < 0.05, **P < 0.01 vs. the corresponding controls