Fig. 5

E2F6/KDM5C-regulated SF3A3 expression facilitates the growth of BC cells. A We predicted the potential E2F6 binding site within the GpC island of SF3A3 promoter using the Ensembl and PROMO 3.0 websites. B ChIP-PCR was used to explore the binding site of E2F6 in GpC island with KDM5C and E2F6 antibodies. C Luciferase reporter assays were performed in T24 cells that were co-transfected with indicated vectors. D, E J82 and T24 cells were transfected with indicated vectors, and then, cell viability was measured by MTS assay. F Cell proliferation was measured by colony formation assays in J82 cells after transfection with shSF3A3 or shROCR1 or both together. G Quantitative analysis of F. All data are expressed as mean ± SEM and come from three independent experiments. *P < 0.05, **P < 0.01 vs. the corresponding controls