Fig. 6

The disruption of the E2F6/KDM5C/SF3A3 axis inhibits BC xenograft progression in vivo. A T24 cells were stably depleted of SF3A3, KDM5C, or both and then injected into nude mice to construct BC xenograft tumors. Tumor volumes were displayed by direct measurement. B Tumor sizes were shown in each group of mice. C The wet weight of xenograft tumors in each group of mice was analyzed. D Double immunofluorescence staining was used to detect SF3A3 and MYC expression in xenograft tumor tissues. E Quantitative analysis of the fluorescence intensity SF3A3 and KDM5C. F The SF3A3, KDM5C, and MYC proteins levels were examined in xenograft tumors using Western blotting. G Quantitative analysis of F. All data are expressed as mean ± SEM and come from three independent experiments. *P < 0.05, **P < 0.01 vs. the corresponding controls