Fig. 2
From: DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35
DDX10 promoted colorectal cancer cell growth and metastasis in vitro. A Expression of DDX10 in several colorectal cancer cell lines by q-PCR; MTT cell proliferation assay was applied. Cell growth rate was significantly suppressed by shDDX10 (B) HCT116 cells (P < 0.001) (C) RKO cells (P < 0.001); D Fluorescence images of HCT116 (P < 0.001) and RKO cells (P < 0.01) were measured via Celigo high-content screening assay. Upper panel was the representative images and lower panel was the growth curve; E Colony formation assays using DDX10‐knockdown HCT116 and RKO cells (P < 0.001); F Cell apoptosis using DDX10‐knockdown HCT116 and RKO cells (P < 0.001); G. Wound-healing assay using DDX10-knockdown HCT116 and RKO cells; H. Transwell migration assay using DDX10-knockdown HCT116 (P < 0.001) and RKO cells (P < 0.01); I. Transwell invasion assays using DDX10-knockdown HCT116 (P < 0.01) and RKO cells (P < 0.001)