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Fig. 5 | Cancer Cell International

Fig. 5

From: A role for the NPM1/PTPN14/YAP axis in mediating hypoxia-induced chemoresistance to sorafenib in hepatocellular carcinoma

Fig. 5

NPM1 regulates PTPN14 localization under hypoxia. A Representative immunofluorescence staining of the localization of PTPN14 and NPM1 in HepG2 cells under normoxia (20% O2) or hypoxia (2% O2) for 24 h. Scale bar = 20 μm. B Total lysate was harvested from HepG2 cells after hypoxia for 0 or 24 h. After the cells had been immunoprecipitated using PTPN14 antibody, their interaction with NPM1 was examined using the indicated antibodies. C Representative immunofluorescence staining of PTPN14 in HepG2 cells transfected with NPM1 siRNA (siNPM1) under hypoxic conditions (2% O2) for 24 h. Scale bar = 20 μm. D The IC50 was measured using an MTT assay after 72 h of sorafenib treatment

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