Fig. 7
NPM1 regulates YAP by retaining PTPN14 in the nucleus under hypoxia. Representative immunofluorescence staining of the localization of YAP in HepG2 cells with NPM1 or/and PTPN14 knockdown (A) and SK-Hep1 cells with NPM1 or/and PTPN14 overexpression (B) under hypoxia (2% O2) for 24 h. Scale bar = 20 μm. C The expression of p-YAP and total YAP in the whole cell lysate (WCL) and nuclear fractions of HepG2 cells with NPM1 or/and PTPN14 knockdown and SK-Hep1 cells with NPM1 or/and PTPN14 overexpression under hypoxia (2% O2) for 24 h was examined by western blotting. D The IC50 was measured using an MTT assay after 72 h of sorafenib treatment. *p < 0.05