Fig. 4

Upregulated-RUFY4 was a prognostic biomarker of ccRCC. A Using Venn plot to pick up genes that are low-expressed in tumors and related to low-density immune-infiltration. B Contrary to A, the figure shows genes that are highly expressed in tumors and related to high-density immune-infiltration. C–F Receiver operating characteristic (ROC) curve of RUFY4, PDCD1, LAG3, and SCL12A1. ROC curves indicate the capabilities of picking up ccRCC patients from the public. AUC means the area under a curve. p-valves were on Figures. G The mRNA levels of RUFY4 in 55 ccRCC tissues and 11 paired adjacent normal tissues in ccRCC based on data from the GEO database. t-testp < 0.0001. H The mRNA levels of RUFY4 in 16 pairs of ccRCC tissues and adjacent normal tissues. t-test, ****p < 0.0001. I The protein levels of RUFY4 in 8 ccRCC tissues and 8 paired tissues in ccRCC. J The protein levels of RUFY4 in HK2 and RCC cell lines. K The mRNA levels of RUFY4 in HK2 and RCC cell lines. t-test, ****p < 0.0001, ***p < 0.)01. L–P The relationship between the expression level of RUFY4 and clinical stage, G grade, T stage, M stage, and N stage. Kruskal-test and Wilcoxon-test were used in statistical analysis. p-valves were on Figures. Q The multivariate survival analysis for the expression level of RUFY4. The red line indicates a high expressing group of RUFY4, and the blue line indicates a low expressing group. HR means hazard ratio and CI means confidence interval. p-valves were on Figures