Fig. 6

GSDM family was involved in promoting metastasis and angiogenesis of colorectal cancer. A These three-circle Venn diagrams showed the interaction between the correlated genes of GSDMA, DSDMB and GSDME. These four-circle Venn diagrams showed the interaction between the correlated genes of GSDMA, DSDMB, GSDME and GSDMD. Notice that there was no or almost no interaction between some groups. The correlated genes of GSDM family members were determined by the Pearson’s Product-moment Correlation test in colorectal cell lines based on Broad Institute Cancer Cell Line Encyclopedia (CCLE) dataset. GSDMA/B/D/E cor (+)/(−) denoted the genes positively/negatively significantly (P < 0.05) correlated with GSDMA/B/D/E. The GO enrichment analysis was performed on the genes correlated with all the four GSDM genes. B GSDME expression was positively correlated with the expression of CD31 and CD34 based on TCGA-COAD dataset analysis. C Increased expression of GSDME provided endothelial development advantage based on Gene Set Enrichment Analysis (GSEA). D Violin plot revealed that GSDME expression was correlated with stage of CRC