Fig. 2

MeCP2 knockdown improved the 5-FU sensitivity of 5-FU-resistant GC cells. Cell viability in GC cells and 5-FU-resistant GC cells treated respectively with 0, 0.1, 0.5, 1, 5, 10, 20, 40, 80 and 160 μM 5-FU for 48 h (A, B). Interferential efficiency of MeCP2 in 5-FU resistant GC cells transfected with siRNA-1 or siRNA-2 targeting to MeCP2, or NC-siRNA, or siRNA-1 or siRNA-2 plus MeCP2-overexpressing plasmid (*p < 0.05, as compared with NC-siRNA; ^#p < 0.05, as compared with siRNA-1 or siRNA-2; Student's t-test) (C, D). Cell viability (E, F), colony formation (G, H), cell cycle (I, J) and cell apoptosis (K, L) were detected in 5-FU resistant GC cells transfected with siRNA-1 or siRNA-2 targeting to MeCP2, or with NC-siRNA, or with siRNA-1 or siRNA-2 + ov-MeCP2, and then treated with 5-FU (*p < 0.001, as compared with NC-siRNA; ^#p < 0.001, as compared with NC-siRNA + 5-FU; Student's t-test)