Fig. 6

MiR-603 played a role in BC by targeting KIF2A. A–L HCC70 and MDA-MB-231 cells were transfected with miR-NC, miR-603, miR-603 + pcDNA, or miR-603 + KIF2A. A, B The levels of KIF2A mRNA and protein in HCC70 and MDA-MB-231 cells were detected with qRT-PCR or western blotting. C–G The cell cycle progression, colony formation, and proliferation of HCC70 and MDA-MB-231 cells were examined with flow cytometry, colony formation, or MTT assays. H–J The migration, invasion, and apoptosis of HCC70 and MDA-MB-231 cells were determined with transwell or flow cytometry assays. K, L Western blotting was conducted to assess the levels of Bax, Cleaved PARP, and Bcl-2 in HCC70 and MDA-MB-231 cells. *P  < 0.05