Fig. 1

Sestrin2 has anticancer effects on CRC cells. A Sestrin2 was downregulated in both COAD (P = 0.28 × 10^–14) and READ (P = 0.024) compared to normal tissue in TCGA. B Cell proliferation was markedly inhibited after Sestrin2 upregulation, as determined by the CCK-8 assay. (*P = 0.05; Mann–Whitney test; connected by medians) C The colony formation assay showed that the number of colonies formed by LV-Sestrin2-infected cells was smaller than that of LV-GFP-infected cells. The right panel shows the number of colonies (*P = 0.05; Mann–Whitney test; lines showed medians). D Cell migration was detected by Transwell assays. The cell number was counted by ImageJ and normalized to the LV-GFP group (right panel) *P = 0.05; Mann–Whitney test; lines showed medians). E Cell migration was measured by the wound healing assay. The area of migration was quantified as the mean ± standard deviation (SD) and normalized to the respective LV-GFP group (right panel); *P = 0.05; Mann–Whitney test; lines showed medians