Table 1 Limiting dilution data showing the effect of Sestrin2 expression on the frequency of CSCs
From: Sestrin2 reduces cancer stemness via Wnt/β-catenin signaling in colorectal cancer
Cells seeded | 1 | 10 | 100 | 1000 | Estimated frequency of CSCs | P value | |
|---|---|---|---|---|---|---|---|
HCT-116 | LV-GFP | 62/78 | 152/162 | 89/92 | 24/24 | 1 in 6 cells | 0.087 |
LV-Sstrin2 | 78/105 | 136/170 | 86/91 | 24/24 | 1 in 7 cells | ||
SW620 | LV-GFP | 45/90 | 101/145 | 76/94 | 24/24 | 1 in 19 cells | 4.24 × 10–8 |
LV-Sstrin2 | 51/128 | 96/172 | 74/96 | 22/24 | 1 in 35 cells | ||