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Fig. 3 | Cancer Cell International

Fig. 3

From: miR-30a targets STOX2 to increase cell proliferation and metastasis in hydatidiform moles via ERK, AKT, and P38 signaling pathways

Fig. 3

miR-30a targets the 3′-UTR of the STOX2 mRNA. A Schematic showing the position of the cloned sequences in the luciferase reporter construct. Below are shown the STOX2 3′-UTR region containing the miR-30a-5p binding site, the miR-30a-5p sequence, and the mutated binding site, used to construct the wild-type and mutant vectors, respectively. B HeLa cells were transfected with wild-type or mutant luciferase reporter vectors with miR-30a mimics to determine the luciferase activity. C and D Western blotting assay to detect the protein levels of STOX2 after regulating of miR-30a expression using mimics or inhibitors. E IHC showing the expression of STOX2 in hydatidiform mole tissues and normal placenta. Bar = 100 μm. *P < 0 .05 or **P < 0 .01, ***P < 0.001

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