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Table 1 EGFR T790M primer information

From: Application of CRISPR/Cas9-based mutant enrichment technique to improve the clinical sensitivity of plasma EGFR testing in patients with non-small cell lung cancer

sgRNA*

Forward

5′-TAATACGACTCACTATAGATCATGCAGCTCATGCCC-3′

Reverse

5′-TTCTAGCTCTAAAACAAGGGCATGAGCTGCATGAT-3′

cfDNA PCR†

Forward

5′-CATGCGAAGCCACACTGAC-3′

Reverse

5′-CGGACATAGTCCAGGAGGCA-3′

  1. sgRNA, single guide RNA; cfDNA, cell-free DNA
  2. *EGFR T790M primer for sgRNA
  3. †Primer for cfDNA PCR. The expected product size was 164 bp
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Cancer Cell International

ISSN: 1475-2867

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