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Table 5 Data of < 10 copies/mL of true positive T790M patient samples

From: Application of CRISPR/Cas9-based mutant enrichment technique to improve the clinical sensitivity of plasma EGFR testing in patients with non-small cell lung cancer

Sample no.

cfDNA qPCR

T790M ddPCR

Detection positive (≥ 2 events/assay)

T790M CRISPR-CPPC assay

Detection positive (≥ 6 events/assay)

Tissue or other samples qPCR

NGS

Result

Events

Wild

Allele frequency (%)

Result

Events

Wild

Allele frequency (%)

Result

Result

Result

3

ND

3

560

0.5

Positive

353

4570

7.2

Positive

L858R

NA

4

Positive

1

238

0.4

ND

102

1811

5.3

Positive

Exon19 del

T790M 2.4%

7

Positive

0

533

0.0

ND

6

1228

0.5

Positive

L858R

NA

8

Positive

3

430

0.7

Positive

7

1556

0.4

Positive

Exon19 del

NA

9

Positive

5

438

1.1

Positive

8

1215

0.7

Positive

Exon19 del

NA

29

ND

3

1170

0.3

Positive

9

2568

0.3

Positive

Exon19 del

NA

30

ND

2

386

0.5

Positive

38

2766

1.4

Positive

L858R

NA

32

ND

0

302

0.0

ND

2

1978

0.1

ND

Exon19 del

T790M from lung tissue

T790M 0.5%

34

ND

1

310

0.3

ND

24

3816

0.6

Positive

Exon19 del

T790M from lung tissue

NA

45

Positive

6

205

2.8

Positive

28

1220

2.2

Positive

Exon19 del

NA

46

Positive

2

114

1.7

Positive

158

7316

2.1

Positive

Exon19 del

NA

48

ND

0

71

0.0

ND

7

2119

0.3

Positive

Exon19 del

T790M 0.1%

54

Positive

0

31

0.0

ND

274

3874

6.6

Positive

L858R

NA

58

ND

0

139

0.0

ND

12

6052

0.2

Positive

Exon19 del

T790M 0.2%

59

ND

0

11

0.0

ND

10

5294

0.2

Positive

L858R

T790M from pleural fluid

ND

60

Positive

1

137

0.7

ND

562

6343

8.1

Positive

Exon19 del

T790M 2.1%

  1. cfDNA, cell-free DNA; CRISPR-CPPC, CRISPR system combined post-PCR cfDNA; ddPCR, droplet digital PCR; NA, not applicable; ND, not detected; qPCR, real-time PCR; SQI, semiquantitative index
  2. *T790M detected by more than two methods (qPCR from cfDNA,tissue or other types of samples, NGS, ddPCR, CRISPR-CPPC assay) simultaneously is considered “true positive”