Fig. 7
Hub lncRNA screening identified TDRKH-AS1 as a key lncRNA in HCC. Expression heatmap of the 55 lncRNAs included in the 50-LPS in the 50 paired tumor and normal liver tissues of TCGA-LIHC cohort (A). Volcano plot of the differential test and the top 6 differentially expressed lncRNAs with log2FC > 1 and FDR < 0.05 were marked in orange (B). Venn diagram identified two lncRNAs (TDRKH-AS1 and MAFG-DT) that were differentially expressed in TCGA-LIHC, CHCC and GSE77509, as well as associated with overall survival in TCGA-LIHC and CHCC cohort (C). The Kaplan–Meier curves of overall survival for TDRKH-AS1 and MAFG-DT in TCGA-LIHC (D, E). Real-time PCR validated the expression difference of TDRKH-AS1 and MAFG-DT in five paired HCC and normal liver tissues (F). *p < 0.05, **p < 0.01, ***p < 0.001. T, tumor; N, normal