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Fig. 3 | Cancer Cell International

Fig. 3

From: miR-223-3p carried by cancer-associated fibroblast microvesicles targets SORBS1 to modulate the progression of gastric cancer

Fig. 3Fig. 3Fig. 3

CAFs-MVs-derived miR-223-3p can facilitate malignant progression of GC. A PKH26-labeled MVs co-incubated with GC cells (PKH26 in red while DAPI in blue) (n = 3, p < 0.0001; p < 0.001; scale bar: 20 μm); B miR-223-3p level in the CAFs (at logarithmic phase, n = 3, p = 0.0237) and MVs of CAFs (n = 3, p = 0.0021) in each transfection group (inhibitor NC, inhibitor); C miR-223-3p level in GC cells after 24 h of MVs co-cultured with GC cells (n = 3; p < 0.001; p < 0.001, n = 3; p = 0.0003; p = 0.0006); D The activity of GC cells after co-culture with MVs in each transfected group (control, inhibitor NC, inhibitor) (n = 3; p < 0.001; p = 0.0028, n = 3; p = 0.0011; p = 0.0214); E The colony-forming ability of GC cells after co-culture with MVs in each transfection group (n = 3; p = 0.0125; p = 0.0459, n = 3; p = 0.0062; p = 0.0023); F The migration and invasion of GC cells after co-culture with MVs in each transfected group (BGC-823 cells: n = 3; p < 0.001; p < 0.001, n = 3; p = 0.0001; p = 0.0003, AGS cells: n = 3; p = 0.0001; p < 0.0001, n = 3; p = 0.0015; p = 0.0007); G Cell apoptosis status (n = 3; p < 0.0001; p = 0.0002, n = 3; p = 0.0003; p = 0.0002); H Cell cycle status (n = 3; p = 0.0106; p = 0.0350, n = 3; p = 0.0101; p = 0.0224); I The expression of EMT-related proteins in GC cells after co-culture with MVs 24 h in each transfected group (BGC-823 cells: n = 3; p < 0.0001; p < 0.0001, p < 0.0001; p = 0.0006, p = 0030; p = 0007, AGS cells: p < 0.0001; p < 0.0001, p = 0.0003; p = 0.0002, p < 0.0001; p < 0.0001). *p < 0.05; **p < 0.01; ***p < 0.001

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