Fig. 4

PGM1 knockdown promoted cell proliferation and arrested more cells in S phase. A PGM1 overexpression markedly inhibited the proliferation of HT29 and SW620 cells, while knockdown of PGM1 markedly promoted the proliferation of HT29 and SW620 cells. B Colony formation abilities of the CTRL, OE-NC, OE-PGM1, shCTRL, and shPGM1 groups of HT-29 and SW620 cells; C EdU assays comparing the proliferation of CTRL, OE-NC, OE-PGM1, shCTRL, and shPGM1 groups of HT-29 and SW620 cells (scale bar = 100 μm). D Flow chart showing the cell cycle distribution of HT-29 and SW620 cells in the five different groups. Data are presented as a mean value ± SD of results obtained from three replicate samples; *P < 0.05, **P < 0.01, ***P < 0.001; ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001