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Fig. 6 | Cancer Cell International

Fig. 6

From: LINC01806 mediated by STAT1 promotes cell proliferation, migration, invasion, and stemness in non-small cell lung cancer through Notch signaling by miR-4428/NOTCH2 axis

Fig. 6

LINC01806 sponges miR-4428 to enhance NOTCH2 expression and activate Notch signaling pathway. A, B The levels of several factors related to Notch signaling pathway were examined via RT-qPCR and western blot in NSCLC cells after LINC01806 was silenced or not. C The binding sequence of miR-4428 and NOTCH2 projected by starBase, as well as the mutant NOTCH2 3’-UTR sequence, was exhibited. D RIP assay disclosed the levels of LINC01806, miR-4428 and NOTCH2 in Ago2-precipiated complex relative to that in control groups. E The luciferase activity changes of NOTCH2 3’-UTR-Wt and NOTCH2 3’-UTR-Mut were assessed via luciferase reporter assay in NSCLC cells with miR-4428 augment. F, G The expression of NOTCH2 was examined via RT-qPCR and western blot in NSCLC cells transfected with sh-NC, sh-LINC01806#1, sh-LINC01806#1 + NC inhibitor or sh-LINC01806#1 + miR-4428 inhibitor. **P < 0.01, n.s. no significance

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Cancer Cell International

ISSN: 1475-2867

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