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Fig. 7 | Cancer Cell International

Fig. 7

From: LINC01806 mediated by STAT1 promotes cell proliferation, migration, invasion, and stemness in non-small cell lung cancer through Notch signaling by miR-4428/NOTCH2 axis

Fig. 7

MiR-4428 down-regulation or NOTCH2 up-regulation abrogates LINC01806-knockdown-induced repression on NSCLC cell proliferation, migration, invasion and stemness. A RT-qPCR was performed to examine the efficacy of NOTCH2 overexpression. B, C The proliferative capacity of NSCLC cells was monitored in colony formation and EdU assays (scale bar = 100 μm) under various conditions (sh-NC, sh-LINC01806#1, sh-LINC01806#1 + NC inhibitor, sh-LINC01806#1 + miR-4428 inhibitor, sh-LINC01806#1 + pcDNA3.1, or sh-LINC01806#1 + pcDNA3.1/NOTCH2). D, E By transwell assay (scale bar = 100 μm), cell migration and invasion under different conditions were tested. F The expression of Oct4, Nanog and SOX2 was detected by western blot in H1299 cells after indicated transfections. G Cell stemness under indicated transfections was assessed by sphere formation assay (scale bar = 100 μm). **P < 0.01, n.s. no significance

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Cancer Cell International

ISSN: 1475-2867

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