Fig. 4

miR-223-3p targets and modulates SORBS1. A Differential expression of GC-related mRNA by analysis in TCGA-STAD; B the intersection between results predicted by 3 databases (Targetscan, miRDB, mirDIP) and the differential genes; C the correlation between miR-223-3p and potential target genes analyzed by ENCORI (p = 2.41e−19); D SORBS1 expression in normal gastric mucosa cells and GC cells (atlogarithmic phase) (n = 3; p = 0.0002; p < 0.0001; p = 0.001); E the binding sites of miR-223-3p and SORBS1 predicted by miRDB database; F Targeted relationship between miR-223-3p and SORBS1 verified by dual-luciferase reporter gene detection (n = 3; p = 0.0092); G Interaction between miR-223-3p and SORBS1 verified by RIP (n = 3; p < 0.0001; p < 0.0001, n = 3; p < 0.0001; p < 0.0001); H SORBS1 level in each transfection group after 48-h transfection (n = 3; p = 0.0367; p < 0.001, n = 3; p = 0.0024; p = 0.0017); I the protein expression level of SORBS1 in each transfection group after 48-h transfection (n = 3; p = 0.0345; p = 0.0068, n = 3; p < 0.0001; p = 0.0073). *p < 0.05; **p < 0.01; ***p < 0.001