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Fig. 4 | Cancer Cell International

Fig. 4

From: DEPDC1B collaborates with GABRD to regulate ESCC progression

Fig. 4

GABRD as a target protein of DEPDC1B in ESCC cells. A The heatmap of DEGs identified by microarray of cells treated with shCtrl (n = 3) or shDEPDC1B (n = 3). B, C The top DEGs with the highest ranking were selected and further validated by qRT-PCR (B) and western blotting (C). D GABRD expression was evaluated in ESCC tissues and normal tissues by IHC. E The effects of GABRD knockdown on Eca-109 and TE-1 cell proliferation in MTT assay. F Cell counting assays to assess the effect of GABRD knockdown on proliferation in Eca-109 and TE-1 cells. G The cell apoptosis was examined by flow cytometry upon GABRD knockdown. H, I wound-healing assay (H) and transwell assay (I) were used for determining migration of Eca-109 and TE-1 cells in shCtrl and shGABRD groups. The representative images were selected from 3 independent experiments, of which 5 fields were randomly selected for counting the cell numbers. **P < 0.01, ***P < 0.001

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