Fig. 5
From: DEPDC1B collaborates with GABRD to regulate ESCC progression
Knockdown of GABRD alleviates the promotion of DEPDC1B on malignant progression of ESCC. A The interaction of DEPDC1B and GABRD in Eca-109 cells were examined by Co-IP experiment. B–F GABRD-knockdown cells and DEPDC1B-overexpressing were constructed to conduct functional recovery experiments to determine the coordinated effect on proliferation (B, C), apoptosis (D) and migration (E–F). G Expression of canonical components of the PI3K/AKT/mTOR signaling pathway was examined in GABRD knockdown Eca-109 cells treated with AKT activator. H–I Apoptosis (H) and migration (I) ability of GABRD knockdown Eca-109 cells treated with AKT activator were examined. The representative images were selected from 3 independent experiments, of which 5 fields were randomly selected for counting the cell numbers. *P < 0.05, ***P < 0.001