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Fig. 4 | Cancer Cell International

Fig. 4

From: KMT2B promotes the growth of renal cell carcinoma via upregulation of SNHG12 expression and promotion of CEP55 transcription

Fig. 4

SNHG12 recruits transcription factor E2F1 to elevate CEP55 transcription. A The correlation between SNHG12 expression and CEP55 expression in RCC samples was analyzed through ENCORI database. B The expression of E2F1 in RCC samples was analyzed through the ENCORI database (RCC = 535, Normal = 72). C The correlation between E2F1 expression and CEP55 expression in RCC samples was analyzed through the ENCORI database. D Cellular localization of SNHG12 was analyzed by fractionation of nuclear/cytoplasmic RNA. E The binding of SNHG12 and E2F1 was detected by RIP. F Detection of E2F1 binding to the CEP55 promoter region by ChIP. G After overexpression of SNHG12, the binding of E2F1 to the CEP55 promoter region was detected by ChIP. H The binding of transcription factor E2F1 to the promoter region of the target gene CEP55 was detected by dual luciferase reporter assay. I: Three sites in the promoter region of CEP55. J The expression of CEP55 in 786-O cells transfected with oe-SNHG12, sh-E2F1 or both was detected by RT-qPCR. *p < 0.05, **p < 0.01. All cell experiments were repeated three times

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Cancer Cell International

ISSN: 1475-2867

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