Fig. 2

Clustering of m6A methylation modification patterns and the biological characteristics and TIME contexture. A The interaction of expression between 23 m6A regulators in PAAD. The m6A regulators in two RNA modification types are depicted by circles in different colors. “Readers”, orange; “Writers”, grey; “Erasers”, red. The lines connecting m6A regulators represent their interaction with each other, and thickness shows the correlation strength between regulators. Negative correlation is marked with green and positive correlation with pink. The size of each circle represents the prognosis effect of each regulator scaled by P-value, and the range of values calculated by Log-rank test are P < 0.001, P< 0.01, P < 0.05 and P < 0.1, respectively. Protective factors for patients' survival are represented by blue and risk factors are represented by purple. B Survival analyses for the two m6A modification patterns based on 176 patients including 127 cases in m6Acluster A, 49 cases in m6Acluster B. (P = 0.024, Log-rank test). C Unsupervised clustering of 23 m6A regulators in the combined cohort. The m6A cluster, survival status, gender, tumor stage, and age were used as patient annotations. Red represents the high expression of regulators and blue represents low expression. D GSVA enrichment analysis showing the activation states of biological pathways in m6Acluster A and m6Acluster B modification patterns. The heatmap was used to visualize these biological processes, and red represents activated pathways and blue represents inhibited pathways. E ssGSEA algorithm; F CIBERSORT approach. The fraction of tumor-infiltrating lymphocyte cells in two m6A modification patterns. Within each group, the scattered dots represent TIME cell expression values. The thick line represents the median value. The bottom and top of the boxes are the 25th and 75th percentiles (interquartile range). The whiskers encompass 1.5 times the interquartile range. The statistical difference of two gene clusters was compared through the Kruskal–Wallis H test. *P < 0.05, **P < 0.01, and ***P < 0.001