Fig. 2
From: Radiotherapy-activated NBTXR3 nanoparticles modulate cancer cell immunogenicity and TCR repertoire
Immunopeptidome profiles obtained by LC/MS/MS analysis. CT26.WT cells were treated overnight with 400µM of NBXTR3, then irradiated with a single dose of 4 Gy. The next day, cells were collected by scraping. MHC-I complex was isolated from 20.106 cells then peptides were eluted and analyzed by LC/MS/MS. Only peptides present in all independent experiments and all groups were presented (Total number = 331) and ranked according to the mean area measured for control group. A Variation of SFVDTRTLL peptide area, according to the tested conditions. This peptide was the most abundant, whatever the condition considered. The sequence of this peptide is related to CO1A2 (Collagen alpha-2(I) chain). B Variation of area for RGPVRGISI, related to RS17 (40 S ribosomal protein S17), TYSPPLNKL, related to TP53 (Cellular tumor antigen p53), SGPERILSI, related to HNRPK (Heterogeneous nuclear ribonucleoprotein K) and AYVPGFAHI peptides areas, related to CUED2 (CUE domain-containing protein 2), according to the condition tested. Area variation of peptides (C) #6 to #100 and (D) #101 to #331, according to the condition tested. Data of independent experiments (n = 3) are represented as the mean of area ± SEM