Skip to main content
Fig. 6 | Cancer Cell International

Fig. 6

From: Radiotherapy-activated NBTXR3 nanoparticles modulate cancer cell immunogenicity and TCR repertoire

Fig. 6

Modulation of TCR repertoire and immune cell infiltrates by NBTXR3 activated by RT. A–C Cell density of CD4+ (A), CD8+ (B) and CD68 + cells (C) infiltrates in treated tumors were analyzed by IHC, 5 days after the last fraction of RT. For each tumor, 3 slices from FFPE blocks (first third, middle and last third of the tumor) were put on the same slide and slices were stained using specific antibodies. Presented data were obtained from four to five mice per group (each dot represents one value). D–F Analysis of TCR repertoire. Box and whisker plot representations of Simpson clonality (D), Morisita index (E) and expanded clones (F) of TCR repertoire sequencing of treated (T) and untreated (–) tumors of CT26.WT bearing tumor mice, three days after the last fraction of RT. Presented data were obtained from seven to eight mice per group (each dot represents one value). Statistical test: One-Way ANOVA test for Simpson clonality and expanded clones, Mann-Whitney test for Morisita index. Bars represent the median. Statistical test: Mann-Whitney test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non-significant

Back to article page