Fig. 8

YOD1 promotes the proliferation and metastasis of pancreatic cancer cells. A The expression of YOD1 in cancer tissues and adjacent cancer tissues of seven groups of clinical samples. N, normal; T, tumor. B The expression of YOD1 between six types of pancreatic cancer cells and normal pancreatic cells (HPDE6-C7). C EMT changes of AsPC-1 and MIA-PaCa-2 cells with YOD1 knockdown or YOD1- overexpression. D, E Transwell assays of 24 h and 48 h were performed to detect the metastasis in AsPC-1 and MIA-PaCa-2 cells with YOD1- knockdown or YOD1- overexpression. Scale bar, 100 μm. F, G EdU incorporation assays were performed to detectcell proliferation in AsPC-1 and MIA-PaCa-2 cells with YOD1- knockdown or YOD1- overexpression. Scale bar, 50 μm. H, I Flow Cytometry was performed to detect cell cycle in AsPC-1 and MIA-PaCa-2 cells with YOD1-knockdown or YOD1-overexpression, and the S-phase fraction reflects cell proliferation. J, K IHC was performed to detect CD3 and CD8 expression in cancer and normal tissues. Scale bar, 50 μm, 400× magnification. The upper right small image is magnified 200× . Blue for pancreatic cells, red for lymphocytes and green for cancer cells