Fig. 2

Downregulation of circ-EPB41 suppressed NSCLC proliferation both in vitro and in vivo. A RT-qPCR detection showing the expression of circ-EPB41 in both A549 and H1650 cells after transfection with sicirc-EPB41 or NC. Data are presented as means ± SD; ^***P < 0.001 vs NC. B Flow cytometry detection showing the percentages of cells in G1, S or G2 phase for both A549 and H1650 cells. C, D CCK8 assays were used to evaluate cell proliferation in both A549 (C) and H1650 (D) cells. Data are presented as means ± SD; ^***P < 0.001 vs NC. E, F Colony formation assays showing proliferation of both A549 and H1650 cells. Data are presented as means ± SD; ^***P < 0.001 vs NC. G In xenograft tumor studies, A549 cells transfected with NC or sh-circ-EPB41 were subcutaneously injected into nude mice and tumor growth curves were plotted. Data are presented as means ± SD; ^***P < 0.001 vs NC. (H) Immunohistochemistry showing the percentage of Ki67-positive cells. The relative levels Ki67-positive cells were calculated. Data are presented as means ± SD; ^***P < 0.001 vs NC