Fig. 6

Downregulation of circ-EPB41 suppressed tumor stemness by regulating the miR-486-3p/eIF5A axis. A–C RT-qPCR detection shows the expression of circ-EPB41 (A–F), miR-486-3p (C, D) and eIF5A. E, F in both A549 and H1650 cells after transfection with NC, sicirc-EPB41, miR-486-3p inhibitor and eIF5A overexpression vector singly or combined. Data are presented as means ± SD; ^***P < 0.001 vs NC; ^###P < 0.001, ^##P < 0.01 vs sicirc-EPB41. G, H Immunofluorescence of OCT-4 in both A549 and H1650 cells