Fig. 7

Downregulation of circ-EPB41 suppressed tumor sphere formation by regulating the miR-486-3p/eIF5A axis. A Images of tumor sphere formation assays in both A549 and H1650 cells (200 cells/well); scale bar, 100 μm. B, C Analyses of tumor sphere formation results indicated that downregulation of circ-EPB41 significantly inhibited self-renewal of NSCLC cells, whereas downregulation of miR-486-3p or overexpression of eIF5A restored self-renewal capacity. n = 10. Data are presented as means ± SD; ^***P < 0.001 vs NC, ^###P < 0.001 vs sicirc-EPB41