Fig. 2

MiR-3189-3p impairs proliferation and migration of TNBC cells. A Plot indicating proliferation of MDA-MB-231, MDA-MB-468, and HME1 cells at the indicated time points. B Cell proliferation assay for mammospheres obtained from MDA-MB-231 cells, performed 72 h post-transfection with the miR-3189-3p or the scramble control. Results are expressed as percent of the number of mammospheres obtained from cells transfected with miR-3189-3p versus the number of mammospheres from control cells. C Representative images of a scratch assay 72 h post-transfection with miR-3189-3p or scramble control. Migration of cells toward the cell-free area was monitored by time-lapse imaging using an Olympus VivaView system. Original magnification 10×. The bar graph represents quantification of the migration obtained from three experiments, each in duplicate. In all graph, the asterisks indicate statistical significance