Fig. 3

MiR-3189-3p impairs migration/invasion of TNBC cells. A Representative images of a transwell migration assay performed using MDA-MB-231 (upper panel) and MDA-MB-468 (lower panel) 72 h post-transfection with the miR-3189-3p or scramble control (Ctrl). Migrated cells were stained with crystal violet and pictures were acquired with an optical microscope. Original magnification 20×. Quantification of the migrated cells was obtained from three different random image fields per insert and averaged from at least three biological replicates. B Representative images of a transwell invasion assay performed using MDA-MB-231 (upper panel) and MDA-MB-468 (lower panel) and analyzed 72 h post-transfection with the miR-3189-3p or scramble control (Ctrl). Transwells were stained and pictures were acquired with an optical microscope. Original magnification 20×. The bar graph indicates the average number of cells migrated through the Matrigel calculated from three different random image fields per insert and averaged from at least three biological replicates. * Indicates statistical significance