Fig. 8
From: Mechanisms of miR-3189-3p-mediated inhibition of c-MYC translation in triple negative breast cancer
Role of 4EBP1 in the miR-3189-3p-mediated c-MYC downregulation. Western blots showing levels of the indicated proteins after silencing of 4EBP1 (A) or eIF4E (B). Cells transfected with the scramble RNA were used as control (Ctrl). Western blot showing c-MYC, eIF4E and 4EBP1 proteins in MDA-MB-231 cells transfected with miR-3189-3p or scramble control in the presence of serum (C) or in serum-free conditions (D). Where indicated, cells were co-transfected with 4EBP1 expression vector. GAPDH was used as a loading control for every blot. E Scatter plot showing proliferation of MDA-MB-231 cells transfected with miR-3189-3p (miR) and either pcDNA3 EV (EV) or 4EBP1. Cells transfected with scramble RNA and EV served as control. * Indicates statistical significance between Ctrl/EV and either miR/EV or miR/4EBP1