Fig. 8

IC2-induced apoptosis depends on the inhibition of SCD1 in MCF-7 cells. A Overexpression of SCD1 alleviated the decrease in the protein levels of PARP and Bcl-2. B, C Quantification of the protein expression levels of cleaved-PARP/PARP and Bcl-2/Bax. D Representative images of DAPI staining at ×200 magnification in MCF-7 cells. Overexpression of SCD1 reduced IC2-induced apoptosis. E Flow cytometry was used to detect intracellular ROS. Overexpression of SCD1 reduced IC2-induced intracellular ROS increases. F Quantification of the level of ROS production compared to the control. Data are presented as the mean ± SEM of three independent experiments (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control