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Fig. 6 | Cancer Cell International

Fig. 6

From: Metallothionein 2A (MT2A) controls cell proliferation and liver metastasis by controlling the MST1/LATS2/YAP1 signaling pathway in colorectal cancer

Fig. 6

Inhibition of MST1/2 rescues the effect of MT2A on CRC cells. A XUM-MP-1 is a specific inhibitor of MST1/2. 2 μM XUM-MP-1 was added and cultured for 6 h. The results showed that both p-MST1 and p-YAP1 were significantly inhibited in HCT8 and HCT116 cells. B, C Colony formation assays showed that XUM-MP-1 promoted colony formation in HCT8 and HCT116 cells as well as rescued the inhibitory effect of MT2A overexpression. D The CCK-8 assay showed that XUM-MP-1 significantly increased the cell proliferation rate of MT2A-overexpressing cells. E IHC staining of MT2A showed that the MT2A overexpression group consistently overexpressed MT2A in subcutaneous tumors. P-MST1 and p-YAP1 were upregulated in the MT2A overexpression group compared to the control group. F Consistent with subcutaneous tumors, IHC staining of liver metastasis tumors showed that MT2A overexpression increased the expression of MT2A, p-MST1 and p-YAP1. Scale bar = 100 μm, OE, overexpression. *p < 0.05, **p < 0.01

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Cancer Cell International

ISSN: 1475-2867

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