Fig. 2

DCs induced by CD40 Ab and OK-432 promoted the proliferation of cytotoxic T cells while suppressing the number of Tregs. A DCs induced by CD40 Ab and OK-432 stimulated the generation of cytotoxic T cells. T cells derived from peripheral blood were treated with different types of DCs induced by relative agents. The combinational group showed relatively more CD8⁺ cells. B DCs induced by CD40 Ab and OK-432 augmented the secretion of IFN-γ. The three agents facilitated the production of stimulatory cytokine IFN-γ. The combinational group displayed an obvious advantage. C Combination of CD40 Ab and OK-432 reduced the number of CD4⁺T cells. Compared with the control groups, the three test groups had a reduced ratio of CD3⁺CD4.⁺ T cells versus total T cells. D DCs induced by CD40 Ab and OK-432 restrained the production of Tregs. The proliferation of Tregs originating from peripheral blood was suppressed in the three test groups, especially the combinational group. E DCs induced by CD40 Ab and OK-432 suppressed the expression of Foxp3. The expression of Foxp3 was obviously suppressed in the three test groups; the effect was significant in the combinational group. Value represents mean ± standard error of mean. P-value represents the comparison of control group versus tested groups